Dna from saliva samples was quantified using a hoefer dyna quant 200 fluorometer and arrayed at a. Dna concentration was measured by dyna quant 200 fluorometer hoefer. The initial number of cfu per milliliter was obtained by plating the 10fold ssw dilutions on hi plates in duplicate followed by incubation at 37c for 18 h. Used hoefer scientific instruments hsi semiphor te70 semi dry transfer unit. Only accessories and parts approved or supplied by hoefer, inc. With the qubit fluorometer, your research is enhanced. To find the total mass of the plasmid cdna, the mass of a. Analysis of promoters recognized by pvds, an extracytoplasmicfunction sigma factor protein from pseudomonas aeruginosa. Ge healthcare amersham biosciences dyna quant 200 dq. Big savings on 200 excellent, buy now at deep discounts. Hoefer dq200 dyna quant 200 fluorometer for sale online ebay. Used hoefer dyna quant 200 fluorometer dna related for.
Read this entire manual before using the instrument and use only according to the instructions. Publications home of jama and the specialty journals of. The ge healthcare amersham biosciences dyna quant 200 dq 200 series fluorometers are designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst 33258 dye. The mass of a single plasmid cdna copy was calculated by the formula. The quantit reagents exhibit high photostability in the qubit fluorometer, showing fluorometer is far more sensitive than uv absorbance, which measures anything absorbing at 260 nm, including dna, rna, protein, free nucleotides, or excess salts. Dyna quant 200 designed specifically for the accurate quantitation of low dna concentrations even in presence of arn, proteins andor nucleotides can also measure enzyme activity based on the cleavage of methylumbelliferyllinked substrates as. Development of a qrtpcr assay to determine the relative. After unpacking the instrument, place the instrument on a flat, level, dry surface. Quantitation of dna using hoechst 33258 biotek instruments. Sperm lysis sperm cells were counted with a hemacytometer, diluted to a concentration of either 0.
Amersham hoefer dq200 dyna quant 200 dna dynaquant fluorometer. Designed specifically for the accurate quantitation of low dna concentrations even in. Technical manual quantus fluorometer operating manual instructions for use of product e6150. If you have any comments on this manual, please send them to us at. We quantitated dna using standardized fluorometric readings on a hoefer dyna quant 200 fluorometer hoefer pharmacia biotech inc, san francisco, calif. Novel gene identified in an exomewide association study of tanning dependence. Dna concentration was determined using a hoefer dyna quant 200 fluorometer. Fluorometer for sale tractor parts and replacement.
Novel gene identified in an exomewide association study. Dna concentrations were determined with a hoefer dyna quant 200 fluorometer as described by the manufacturer amersham pharmacia biotech. Sodium bisulfite modification genomic dna from freshfrozen lung cancer was modified using a method described previously. Dq200 dq manual with fluorometer hoefer 200 dq with hoefer dq200 200 manual fluorometer hoefer dq. Purified genomic dna was digested with the same restriction enzyme as that used for generation of the crosslinked template, and restriction fragments were randomly ligated without dilution using a dna concentration. Taqman pcr for detection of vibrio cholerae o1, o9, nono1. Hoefer dyna quant 200 fluorometer, model dq200115v, is designed specifically for the accurate quantitation of small volume samples with low dna c. Each sample was diluted to a final concentration of 5 ng. Simple sequence repeat diversity of a worldwide collection. Dyna quant 200 fluorometer is designed specifically for the accurate quantitation of small volume samples with.
Measurement of dna concentrations using hoechst 33258. It will guide the user through all the measurement steps. The dyna quant 200 fluorometer is designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst 33258 dye. Pharmacia biotech, san francisco, ca, usa was developed for the quantification of dna with the h33258 fluorochrome. List names where possible all occupants of lab g10 and users of the hoefer dyna quant 200 fluorometer 3. This fluorometer boots up, passes all self tests, responds to keypad input and zeros as i. For the best chance of winning, increase your maximum bid. Total genomic dna was quantified using a fluorometric assay provided by a hoefer dyna quant 200 fluorometer hoefer pharmacia biotech inc. Using the dilution calculator feature of the qubit 2. The dna concentrations of the pcld04541 and pbelobac11 clones were also measured with a dyna quant 200 fluorometer hoefer using the dnaspecific binding dye hoechst 33258 sigma, st louis, mo to stain the dnas. Genome amplification of single sperm using multiple. Attach copies of data sheets name amount hazard risk phrases hazdat no biosciencesno. The dye binds preferentially to doublestranded dna, thus allowing accurate quantitation. Measurement of dna concentrations using hoechst 33258 dye in.
Renishaw ramascope dualwavelength microraman spectrophotometer. The quantus fluorometer is a dualchannel fluorometer for your personal quantitation workflow. Development of a qrtpcr assay to determine the relative mrna. The dna concentration was determined using a dyna quant 200 fluorometer hoefer. Cloning and characterization of pvds, a gene required for pyoverdine synthesis in pseudomonas aeruginosa. Fluorometer used fluorometers for sale with warranties and worldwide shipping lab fluorometer. Labtrader is becoming premier biotech equipment same great equipment, service, and staff. Genetic associations with tanning dependence td have not yet been explored. Aug 02, 2006 the dsdna binding dye the accurate determination of nucleic acid con hoechst 33258 used in conjunction with the centration in solution is a critical operation in hoefer dyna quant 200 fluorometer can detect as little as 20 ng dna in a 2 ml sample teare j. Laboratory equipment manuals hitechtrader used lab equipment. Dna from pure cultures of azoxystrobinresistant alternaria isolates 37e3 and 37b8, and sensitive isolates a039 and 3a2, and from healthy pistachio leaves was also extracted by the fastdna kit and quantified using the hoefer dyna quant 200 fluorometer hoefer pharmacia biotech. Model tko 100 hoefer tko 100 dna fluorometer tko 100 hoefer scientific instruments dna fluorometer model tko 100 hoefer tko100 dna fluorometer used nice. You need to be registered and approved to bid at this auction.
The fluoroselect fluorometer can be powered with 4 aa alkaline batteries or a power adapter 5vdc. This fluorometer boots up, passes all self tests, responds to. The dyna quant 200 fluorometer is designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst. The invitrogen qubit microrna assay kit is designed for easy and accurate quantification of small rna 1725 nt or bp such as microrna, even in the presence of common contaminants such as salts, free nucleotides, solvents, detergents, and protein. After a flash of onoff welcome screen, similar to the one shown in figure 1, the screen automatically. Another approach quantification using a fluorometer, such as the dyna quant 200is recommended for. Fluorometer function and description the hoefer dq 200 fluorometer is a. Moreover, uv spectrophotometry is often not sensitive enough to accurately measure low concentrations of dna and rna. The assay is highly selective for small rna over rrna or large mrna 1,000 bp. The hoefer dyna quant 200 fluorometer, model dq200115v, is designed specifically for the accurate quantitation of small volume samples with low. Hoefer dyna quant 200 flourometer analytical instruments llc. Hoefer he100 he 100 supersub electrophoresis manual.
Publications home of jama and the specialty journals of the. Measurement of dna concentrations using hoechst 33258 dye in the hoefer dyna quant flourometer. For a current list of dyna quant 200 application notes see ordering information in the customer service information section of this manual. We have been involved in the resale of surplus items for over three decades. Measurement of nucleic acid concentrations using the dyna. Fluorometer for sale pool, patio, and bbq supplies. Universal resource trading limited is a specialist sales company in the scientific industry but we also work in antiques and other unique items. Designed to provide highly sensitive fluorescent detection when quantifying nucleic acids and proteins, the compact instrument is simple to operate. After isolation, the concentration of the plasmid cdna was determined in a hoefer dyna quant 200 fluorometer amersham pharmacia. Similar results were also observed for pslj1711 clones versus pbelobac11 clones. The hoefer dyna quant 200 fluorometer is designed specifically for measurement of low dna concentrations using the hoechst 33258 dye in low volume samples. Hoefer pharmacia biotech red rotor laboratory platform shaker. Used hoefer dyna quant 200 fluorometer dna related for sale. Hoefer dna fluorometer model tko 100 hoefer tko 100 dna fluorometer tko 100 hoefer scientific instru.
Dyna quant 200 designed specifically for the accurate quantitation of low dna concentrations even in presence of arn, proteins andor nucleotides can also measure enzyme activity based on the cleavage of methylumbelliferyllinked substrates as well as. The instrument has a narrowband excitation light source. Martinique elevated pet diner walnutblack 1 quart, hoefer dyna quant 200,dashcam gps wifi fullhd auto kamera hd 1080p,170 grad weitwinkel neu top. Fluorometers are used to gauge the factors of fluorescence, such as its intensity and wavelength distribution of the emission spectrum, after excitation by a certain spectrum of light. Hoefer tko100 dna fluorometer quantitative analysis. Hansatech modulated fluorimeter or fluorometer mfms fms. Plug one end of the supplied power cord into the qubit 2. Ssllc offers a wide selection of used electrophoresis for your lab. The hoefer dyna quant 200 fluorometer, model dq200115v, is designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst 33258 dye. The instrument must always be used with the earth lead of the power cord correctly grounded to earth at the mains outlet. Your bid or registration is pending approval with the auctioneer. Eleven ssr primer pairs were used in this study table 2. Find hitachi, thermo fisher scientific, and shimadzu for sale on machinio.
Amersham biosciences except for alterations described in the user manual. Pharmacia biotech hoefer dyna quant 200 fluorometer model dq200unit powers on. Hoefer scientific pr electrophoresis gel membrane processor 115v. This fluorometer boots up, passes all self tests, responds to keypad input and zeros as i think it should. Hoefer gd 2000 vacuum slab gel dryer gd2000 tested. Hoefer dyna quant 200 fluorometer instructions hitachi model 10080 computerized spectrophotometer hp 5890 series ii gas chromatograph reference manual, site prepinstallation manual, and tutorial hp 5989a ms engine site preparation manual hp 7673 automatic sampler installing the hybrid injector bracket manual. This instrument is designed for indoor laboratory use only. Thermo fluoroskan ascent microplate fluorometer 374 working condition.
Universal resource trading ltd used lab equipment dealer. A realtime pcr assay for the detection of azoxystrobin. Measurement of nucleic acid concentrations using the dyna quant tm and the genequanttm. Laboratory equipment manuals hitechtrader used lab. Development of a qrtpcr assay to determine the relative mrna expression of two different trypsins in atlantic cod gadus morhua. Sep 10, 20 the dyna quant 200 fluorometer is designed specifically for the accurate quantitation of small volume samples with low dna concentrations using the hoechst 33258 dye. Sort by beckman du7500 diode array spectrophotometer. Taqman pcr for detection of vibrio cholerae o1, o9, non. Analysis of fto gene variants with obesity and glucose. Hoefer dyna quant 200 flourometer analytical instruments. Designed specifically for the accurate quantitation of small volume samples with low dna.
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